When analyzing substrates in cultivation media, it is necessary for a sample to be removed from the bioreactor (fermenter) under sterile conditions. The reproducibility and relevance of the sample taken must remain intact during this step.
Both procedures enable safe and sterile removal from the bioreactor.
The simplest method is to directly measure a filtered sample of medium. However, because reactor medium is used, the range of applications is limited to processes for which there's a sufficient reactor volume or which allow continuous-feed.
Dialysis sampling is an option when processes are involved for which reactor volume does not allow enough sample material. This method only removes low molecular substances from the reactor medium, without reducing the volume of fluid.
TRACE is using a combination of immobilized enzymes and electrochemical transducers for the production of biosensors. This combination is extremely robust and versatile. The biosensor is mounted in a flow-through cell, see figure. This results in a compact measurement cell which can be preconfigured in tubesets for specific applications.
The enzymatic determination of glucose is performed according to the following reaction:
Similarly, the determination of lactate:
Depending on the application, the service life of the sensor system ensures 30 days or 5,000 measurements. The deviation from the average measurement is less than 1.5% for a measurement of 2 g/L glucose and 1 g/L lactate.
Are you interested in a product from us?